Notes
Some potential plans for current stage:
Deal with the poor specificity
decrease the conc. of DMF, even try dd H2O
try to add a lower conc. of pyrene-butanol in the second step
higher hybridisation temperature
another blocking reagent? (other polycyclic aromatic compounds)
another immobilisation mechanism?... (covalent)
Investigate pi-pi stacking using DNA oligos. (determine the protocol like conc. and incubation duration...)
Functionalise electrodes using spotter machine
Mind clearing: 还是很想坚持再优化一下旧的protocol，可能再给自己一点时间到六月。同时多看一些covalent binding相关的文章，在六月之后实在不行就shift到covalent binding。graphene 上的pi-pi stacking 还是值得研究，毕竟oligo都买了对吧。。。 可以从五月中旬开始（一个月以后）做相关的实验，在这之前先把protocol 确定一下，看一些别人怎么研究pi-pi stacking 文章（maybe在别的材料上。。。）
https://www.sciencedirect.com/science/article/pii/B9780123822390000169
this chapter reviews the various types and properties of these carbon allotropes and provides protocols for successful modification or conjugation using a number of reagents and reaction strategies.
Questions for panel discussion:
Will AI take place some job positions in design and creation industries?
How to deal with situations like an artist recreating a piece of art originally generated by AI? Who owns the copyright? Will this be a trend or even a main way of artwork in the future?
Is there an ultimate form of AI? what will it be like? Will human beings evolve to be more intellegent in this competition with AI or will our intellegence be deprived because of laziness?
What do you think of the relationship between AI and human beings? Is it supplementary, a collaboration or a competition?
这个图做的不错，可以参考一哈
https://doi.org/10.1021/acssensors.1c00851
plan1 （Fri to Mon）
plan2 （Sat to Mon）
plan3：Fri（爱丁堡到巴黎）
Mon （巴黎到格拉 两个时间选项，第二个时间可以多玩会）
Dear customer service team,
Because I had to reschedule my trip to Brussels, I informed the hotel I would have a delayed check-in on the 4th of May and only stay for one night. I completely understand that I made a nonrefundable booking and didn't ask them to return my money. However, they not only did not reply to my request but also cancelled my whole booking without any other explanation.
To my understanding, even though I wouldn't show up for the first few days, I still have the right to keep my room because I had paid. Not to mention I've told them I will go to stay for one night. I think what they've done is very unfair and impolite. I want to have at least one-night money back or give me access to write my review (because of the cancellation, I can't review on them)
If you could help me with this I would be super grateful. Thank you.
Mont des arts
For tutor or demonstrator positon
Personal statement
I am a first-year PhD student specializing in electrochemical biosensors and diagnostics. Previously, I pursued an MSc in Biomedical Sciences and continued on to pursue my PhD in the same field at this remarkable university. I am well-versed in medical and biomedical sciences, laboratory science, and practical skills. Given the opportunity, I would be thrilled to contribute to teaching and the marking process.
During my undergraduate studies in China, I achieved an average grade of 3.5. Subsequently, I completed my MSc in Biomedical Sciences by research with Distinction in UoE. I also underwent one year of training at a hospital where I gained experience in Clinical Laboratory, Haematology, Blood Transfusion, and the Emergency Department. In these settings, I employed biochemistry, microbiology, immunology, and molecular biology techniques to analyze various body fluid samples. Moreover, I am proficient in operating a variety of biochemistry analytical instruments, including the BD FACSCanto II flow cytometer, VITEK MS, Beckman Coulter AU 480 chemistry analyzer, and Roche LC480.
Throughout my academic journey, I have actively participated in diverse research topics. During my undergraduate studies, I focused on proteomics and published a review article as the first author (https://doi.org/10.1016/j.biochi.2021.06.006). In my Master's program, I shifted towards interdisciplinary research, with both of my MSc projects involving the development of point-of-care biosensors. Additionally, I contributed to a study on liver cold storage, which was published in Science Translational Medicine last year (https://doi.org/10.1126/scitranslmed.abj4375). As a result of these research experiences, I am proficient in biological experiments such as cell and bacteria culture, ELISA, H&E staining, immunofluorescence (IF), immunohistochemistry (IHC), PCR, and Western blotting (WB). Furthermore, I have expertise in electrochemical experiments like electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV).
Regarding my teaching experience, I have provided private lessons to pre-high school students in subjects such as chemistry, physics, and biology. Typically, I taught two or three students per lesson. My approach involved presenting them with several questions prior to the lecture, encouraging them to utilize available resources such as textbooks and the internet to solve problems. Before the lecture, I allocated 10 minutes for them to discuss their answers with each other. Subsequently, I delivered the lecture which would cover all the key points and highlighted content related to the given questions. This approach facilitated students' awareness of what they should focus on. Most students remained engaged and active throughout the lesson, as their curiosity was piqued. By solving problems independently, they were better able to retain the knowledge.
Additionally, I have honed my communication skills through volunteering. For instance, I assisted in organizing the "AI & Art" theme for the Creative Reaction event in Pint of Science 2023. As part of my responsibilities, I liaised with the main speakers.
I anticipate that this position will provide me with further teaching opportunities, aligning with my career goal of remaining in academia, sharing my research with the public, and imparting knowledge to future generations. I firmly believe that teaching benefits both educators and students. Thank you for considering my application.
All experssions for
+ ions: cation, cathodic,
-inons: anion, anodic
''knowing about a virus does not inherently make us more prepared ''
“We have to be clear what is for today—actions here and now to save lives—versus what is for generating knowledge.''
https://asm.org/Magazine/2022/Fall/Hunting-for-the-Next-Pandemic-Virus
Graphene oxide (GO) is a two-dimensional nanosheet, which is known for its possession of a large surface area and excellent biocompatibility with biomolecules such as DNA, cells, antibodies, and other proteins. (13,14) GO has a largely hydrophobic basal plane with various oxygen-containing functional groups such as epoxides, carbonyl, carboxyl, and hydroxyl groups. (15) Thus, protein molecules can interact with GO via noncovalent interactions such as electrostatic interaction, hydrogen bonding, hydrophobic interaction, and π – π stacking interaction. However, GO hardly interacts with dsDNA. (13,16−18) The incorporation of biological molecules with GO has extensive potential in the development of biosensors. In addition, GO can quench the fluorescence via fluorescence resonance energy transfer (FRET). (15,19−25) The fluorescence signal of fluorophores can be quenched by GO in proximity and the effective quenching distance can be extended to ∼30 nm. (19,26)
travel plan
Edi to Nice 18-21 price: £199
A Q&A to chatGPT
Q: What do you think the probable reason that different bases of nucleobases have various ability to bind to graphene (pi-pi stackingly)?
A:
The ability of different nucleobases to bind to graphene through pi-pi stacking interactions can be attributed to several factors, including the molecular structure of the nucleobases and the nature of graphene itself. Here are some key reasons why different nucleobases exhibit varying abilities to bind to graphene:
Aromaticity: Nucleobases contain aromatic ring structures, which are electron-rich regions that can engage in pi-pi stacking interactions with graphene's conjugated pi electron system. The strength of this interaction depends on the extent of aromaticity in the nucleobase. For example, purines (adenine and guanine) and pyrimidines (cytosine and thymine) all have aromatic rings, but the size and arrangement of these rings differ, leading to variations in binding affinity.
Electron Density: The electron density distribution within the aromatic ring of a nucleobase plays a crucial role in pi-pi stacking interactions. Nucleobases with higher electron density in their aromatic rings tend to form stronger pi-pi interactions with graphene. For instance, guanine has a higher electron density than adenine, making it more prone to pi-pi stacking with graphene.
Geometry and Size: The three-dimensional structure of the nucleobase, including the orientation of the aromatic rings and the distance between them and the graphene surface, can affect the strength of pi-pi stacking. Nucleobases with favorable geometric configurations for stacking tend to bind more strongly.
Functional Groups: Some nucleobases have additional functional groups that can participate in non-covalent interactions with graphene. For example, guanine contains a carbonyl group in addition to its aromatic ring, which can contribute to pi-pi stacking interactions.
Solvent Conditions: The surrounding solvent environment can influence pi-pi stacking interactions. Changes in solvent polarity, temperature, and ionic strength can affect the strength of binding between nucleobases and graphene.
Graphene Properties: The quality and functionalization of the graphene surface can also impact pi-pi stacking interactions. Defects or functional groups on the graphene surface can enhance or inhibit binding with nucleobases.
In summary, the ability of different nucleobases to bind to graphene through pi-pi stacking interactions is a complex interplay of molecular structure, electron density, geometry, and environmental factors. This diversity in binding affinity can have implications in various fields, including materials science and the design of DNA-based nanomaterials. Researchers continue to study these interactions to better understand and manipulate them for practical applications.
When I ask ChatGPT what is click chemistry
Click chemistry is a term coined by chemist K. Barry Sharpless in the early 2000s to describe a set of highly efficient and reliable chemical reactions used for the rapid and selective synthesis of compounds. Click chemistry principles are widely applied in various fields of chemistry, biology, and materials science.
The key features of click chemistry reactions include:
High Yield: Click reactions typically have high yields, meaning that a large proportion of the starting materials is converted into the desired product.
Orthogonality: Click reactions are often orthogonal, which means they can be carried out in the presence of other functional groups without interfering with them. This is crucial for the selective modification of complex molecules.
Modularity: Click reactions are modular, allowing researchers to combine various building blocks or components to create complex molecules, polymers, or materials with precision.
Readily Available Reagents: Click chemistry reactions usually employ readily available and inexpensive reagents, making them practical and cost-effective.
Mild Reaction Conditions: Click reactions are typically conducted under mild conditions (e.g., at room temperature or with minimal heating), reducing the risk of side reactions or damage to sensitive compounds.
Two of the most well-known click chemistry reactions are:
1. Huisgen 1,3-Dipolar Cycloaddition: This reaction involves the formation of a 1,2,3-triazole ring through the reaction between an azide and an alkyne functional group. Copper(I) catalysts are often used to facilitate this reaction, making it one of the most commonly employed click chemistry reactions.
2. Copper-Catalyzed Azide-Alkyne Cycloaddition (CuAAC): In this reaction, an azide and an alkyne react in the presence of a copper(I) catalyst to form a 1,2,3-triazole linkage.
Click chemistry has found applications in various fields, including drug discovery, materials science, bioconjugation, and chemical biology. It enables the efficient and precise synthesis of molecules and materials, making it a valuable tool for researchers in these disciplines.
-0.3 V electric field was applied to attach PASE onto graphene
Would it be helpful in my case?
Penta-graphene
Not like graphene which needs to be functionalized for opening a band gap
When I ask ChatGPT what does 'band gap' mean to a material, for example, graphene
Answer:
The energy gap between valence band and conduction band of a material
Valence Band: The valence band is the range of energy levels in a material where electrons are typically found in their lowest energy states. Electrons in the valence band are tightly bound to atoms and are not free to move around the material to carry an electric current.
Conduction Band: The conduction band is the range of energy levels just above the valence band. Electrons in the conduction band have higher energy and are free to move through the material, allowing them to carry an electric current.
Band Gap: The band gap is the energy separation between the valence band and the conduction band. In materials like insulators and semiconductors, there is a relatively large band gap, which means that it takes a significant amount of energy to move an electron from the valence band to the conduction band. This makes these materials poor conductors of electricity under normal conditions because very few electrons have enough energy to bridge this gap.
Now, specifically in the case of graphene:
Graphene is a unique material in that it is a single layer of carbon atoms arranged in a hexagonal lattice. It is a zero-band-gap semiconductor, meaning it has no energy gap between its valence and conduction bands. Electrons in graphene's structure are described as "massless" Dirac fermions, and they behave more like relativistic particles. This characteristic gives graphene its exceptional electronic properties, such as high electron mobility and excellent electrical conductivity.
In summary, the band gap of a material like graphene refers to the energy difference between its valence and conduction bands. Graphene itself is distinctive because it has a zero-band-gap, allowing electrons to move freely and exhibit unique electronic properties.
What is my life-goal tree
Top tier: A happy happy family, a free free life (free of money concer, health concer, society concer)
midium tier: Finish my PhD successfully, possibly persume a second degree (management, policy making, law, art... )
Base tier: protect enviroment, publish my main work and side work (ACS, RCS), gain more career experience (demonstrator this year, tutor next year), improve my langauge (Chinese, English, Japanese)
I'm soooo proud of myself!! I analysed these data on by using python for the first time!!!!
Although the result of the candidate blocking reagent is shity...